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Chapter 13 Blog: Gene Regulation (Kimberly)

Page history last edited by Kimberly Balao 13 years, 4 months ago

In the first section of this page, you will write a daily summary of that day's class.  For example in  your chapter 2 blog, your first entry should be titled 9/3/10.  You should then write a one or two paragraph summary of that day's lecture, outlining the major points.  In the second section, you are required to add two items (link to a website, video, animation, student-created slide show, student-created PowerPoint presentation) and one journal article pertaining to a topic in this chapter.  A one-paragraph summary must accompany each item describing the main idea and how it applies to the lecture topic.  Please see the PBWorks help guide for assistance embedding video and other items directly in the page.  I will also produce a how-to video on using tables to wrap text around items and other useful tips.  Please see the syllabus for organization and grading details.


A.  Chapter Blog


Chapter 13 was all about Gene Regulation. 


Gene Regulation (The ability to control levels of gene expression) is essential to the life of living organisms. When one regulates genes, they conserve energy by having the ability to turn on or off the synthesis of new proteins, they are a big factor in cell differentiation and also a big factor on how living organisms respond to their environment. Gene Regulation is differs from prokaryotes to eukaryotes. Prokaryotes have operons, which are a cluster of genes, usually with the same function that can be regulated simultaneously. They accomplish this by having multiple ribosome binding sites and long mRNA. Gene Regulation in eukaryotes were more intricate than prokaryotes and they also lacked an operon meaning that genes with similar functions still need to be regulated individually. Not one structure plays a big role in gene regulation in eukaryotes. They have something called combanitorial control in which activators or repressors stimulate the function of RNA POL II or methylation. 


With the homework, I had really hard time trying to find out the lac operon mutations and how they would be expressed in the presence or absence of Lactose. So, note to self, talk to Dr. Weber about that.....



B.  Useful Materials


This is a video on a trp Operon. This operon is a repressor that encodes enzymes required to synthesize the amino acid tryptophan. Tryptophan is responsible for activating the repressor. This video is showing you the model of the operon. I had difficulty trying to imagine this structure in my head so they begin to put the structures in order and in different colors to show differentiation. They show how the repressor binds to the promoter and prevents transcription via RNA Polymerase. 


This video further explains lac operons and it visually helps you sequence the order of events in the lac operon. Lactose is the inducing agent the deactivates the repressor. I also learned that permease is a membrane bound protein that increase the transport of lactose into the cell. The repressor is ultimately activated again when there is low amount os lactose concentration in the cell.  



"Increased Slug and Decreased E-cadherin Expression is Related to Poor Prognosis in Patients with Gastric Cancer"


This Pubmed study about how Increased Slug, a transcriptional factor that represses the transcription of the E-cadherin gene can ultimately hinder the early detection of gastric cancer.E-cadherin is connected to the progress metastasis and gastric cancer. They wanted to know if there was a connection between these two. So, they took patients and tested them for these kinds genes in their gene expression. Turns out, they were really connected. People with high Slug and reduced E had a greater lymph node metastasis. People with normal E or negative Slug had better outcomes. The conclusion was basically that doctors should also look at levels of Slug to help determine the level of gastric cancer they are in.  



Comments (3)

Derek Weber said

at 11:38 pm on Feb 15, 2011

When are we going to talk about these lac mutations?

Derek Weber said

at 11:39 pm on Feb 15, 2011

What about splicing in your summary? It is too short and is missing some major ideas. Use the learning objectives as your guide.

Derek Weber said

at 11:40 pm on Feb 15, 2011

I like the journal article and the summary. Nice job.

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