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Chapter 4 Blog: General Features of Cells (Semon)

Page history last edited by Semon Rezchikov 13 years, 8 months ago

In the first section of this page, you will write a daily summary of that day's class.  For example in  your chapter 2 blog, your first entry should be titled 9/3/10.  You should then write a one or two paragraph summary of that day's lecture, outlining the major points.  In the second section, you are required to add two items (link to a website, video, animation, student-created slide show, student-created PowerPoint presentation) and one journal article pertaining to a topic in this chapter.  A one-paragraph summary must accompany each item describing the main idea and how it applies to the lecture topic.  Please see the PBWorks help guide for assistance embedding video and other items directly in the page.  I will also produce a how-to video on using tables to wrap text around items and other useful tips.  Please see the syllabus for organization and grading details.


A.  Daily Blog

9/22/10: Today in class, we discussed basic cellular structure. Oh, joy. This was, naturally, all review for everyone, since we had basic cellular structure drilled into our heads quite well 2 years ago. More interestingly, Dr. Weber discussed how the ER->Golgi->Membranes pathway was determined. Turns out it was a clever experiment called a "pulse-chace-experiment", which used H3-leucine as the tracer. I found that very interesting, seeing how we figured these results out!

The class also failed at learning, it seems. No one remembered the basics of protein trafficking; I kind of remembered it, but was bad at wording what I knew. I don't mind the idea of only having 1-attempt homeworks; I think that would force people to actually read the book.

Side note: My friend linked me to this article today. I thought it was interesting. I didn't read the study papers themselves, but the results make sense.


9/24/10: I was a bit late to class today, but I was came in just in time to hear about the FREE IPOD TOUCHES THAT WE WILL BE GETTING, COURTESY OF DR. WEBER'S AWESOME COMITEE *cough manipulation cough* SKILLS. Unfortunately, we won't be getting them for personal use, but instead for in-class textbook access, which is still pretty cool. The rest of class was basically a quiz on protein trafficking. Dr. Weber discussed how cotranslational sorting works, giving us the actual binding sequence for SRP and having us calculate whether it would be hydrophilic or hydrophobic. It amazes me a little that simply hydrophobic interactions are enough for SRP to bind strongly to the peptide; I would have thought that a molecule that has to drag a group of very large molecules all the way to the ER would have to bind to its passengers more strongly. Also, I liked how Dr. Weber proposed a "completely hypothetical" scenario where there was a tiny mutation in the beginning of a Ca+ regulation gene, which meant that the protein would stay in the cytosol and be degraded, which meant that the cell would explode due to osmosis; and then, for the big surprise, Dr. Weber revealed (to put it in internet-speak) that this scenario is completely hypoth-IT'S ACTUALLY CYSTIC FIBROSIS LOLOLOL.

I was amused.

B.  Useful Materials


Quite simply, this article explains how protein trafficking in chloroplasts, using the chloroplast analogues of SRP, works. On an interesting note, chloroplasts lack an RNA component to their SRP, which is strange.



One of the papers that suggests that SRP problems lead to cystic fibrosis. Seems to use some interesting techniques for analyzing proteins in detail.



It looks like pulse chase analysis is used for a lot of interesting things. Like, for instance, in vivo phage assembly kinetics. Because of pulse chasing, they changed the model for T4 phage tail building! Hurray for the progress of science!



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